Effect of Zinc Oxide Nanoparticles Biosynthesized by Leuconostoc Mesenteroides ssp. Dextranicum Against Bacterial Skin Infections

The current study included a unusual method for the biosynthesis of ZnO nanoparticles using Iraqi isolate a probiotic bacteria Leuconostoc mesenteroides ssp. dextranicum. The characterizations of ZnO nanoparticles were performed by Xray diffraction (XRD) Atomic Force Microscopy (AFM) technique, Scanning Electron Microscopic (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and Energy-dispersive X-ray analysis (EDX) spectra. The results confirmed that the obtained ZnO nanoparticles are well dispersed, stable and spherical with maximum particles in size range within 37-41 nm in diameter. The aim of this study was to determine the antibacterial and antivirulence factors properties of biosynthesized ZnO nanoparticles against bacterial skin infections including Acinetobacter baumanii ,Klebsiella pneumonia, Pseudomonas aeruginosa and Staphylococcus aureus. The antibacterial activity was evaluated through the determination of the minimum inhibitory concentration (MIC), the results obtained suggested that the MIC ranged between (25 -50) mg/mL and the anti-virulence factors also determined against hemolysin, urease , pyocyanin production and biofilm formation of tested bacteria. Results showed that the ability of hemolysin, urease and pyocyanin production were decreased after treatment with biosynthesized ZnO nanoparticles, also the best effect has been shown in biofilm formation after 72 h for all isolates, with high inhibition 48.74% against Staphylococcus aureus (S9) .