Quantitative phase imaging of MG63 cancer cells for monitoring changes in morphology with time using spatially low and temporally high coherent light source

In the present work, we have synthesized pseudo- thermal light source and developed the speckle-free quantitative phase interferometric microscope which can be utilized to determine various biological parameters such as morphology, phase, refractive index, cell density etc. We have obtained the phase images of the cultured MG63 cancer cells at different time points for the quantification of different cell stages after seeding. Small changes in the morphology cannot be distinguished using bright field and fluorescence microscopy. But the technique presented in this paper, can quantify morphology and shape of the cell easily.