A Flow-Through Device for Simultaneous Dielectrophoretic Trapping and AC Electroporation

Cell isolation and selected transfection of those isolated cells is integral to single-cell analyses platforms. We present a scalable platform for simultaneous negative dielectrophoretic trapping and AC electroporation of cells and experimentally validate that cells can experience both phenomena simultaneously in a controlled manner. The geometry is scalable for single-cells. This has the potential to address two primary problems facing microscale electroporation: cell proximity to coplanar electrodes and material proximity to porated cells.