Comprehensive, High Throughput Screening of Neuron Behavior on Gradient Micro-Alignment Topographies

Microfabrication techniques have improved the ability to create platforms where neuronal cells can be grown and guided to form connections in vitro and in vivo. In particular, regulating neuronal growth throughout maturation, and constructing effective neural circuitry, will increase understanding of the neuron-microenvironment interaction. In this work, the development and fabrication of a linear, anisotropic gradient microridge/groove array, using a PDMS micromolding technique, will be described. This allows more efficient analysis of neuron cells in variable mircoenvironments. Immunofluorescence staining of hippocampal neurons is implemented to compare the morphological and synaptic network changes induced across the gradient geometry.