Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis

Inherent left-right (LR) asymmetry is commonly seen in tissue/organ microarchitecture. However, how to reconstitute such tissue morphogenesis remains elusive. Here we report a method to recapture the inherent LR asymmetry during myogenesis. C2C12 skeletal myoblasts were cultured and induced on micropatterned cell-adherent stripes with different stripe width ranging from 20 to 800 μm. Analysis of cell orientation revealed a significant LR bias on stripes with width of 50 and 80 μm before and after myogenic induction. Moreover, cells on such stripes also exhibited increased myotube formation, suggesting an optimized experimental condition for higher degree of myogenic differentiation with inherent LR-biased cell orientation. The result pave ways for reconstructing LR asymmetry in tissue morphogenesis in the future.