Synchronous fluorescence spectroscopy of soft tissues – tool for diagnostics of malignant lesions

Different steady-state fluorescence spectroscopy approaches are investigated to obtain the highest quality spectral information from biological objects. Synchronous fluorescence spectroscopy (SFS) leads to improved spectral resolution in detection of multi-compound and complex systems, where several fluorophores with similar excitation and emission properties have place. In this study SFS was used to detect and differentiate normal and neoplastic skin and gastrointestinal lesions.