3D electrodes integrated in a dynamic micro array for liposome electrofusion

This paper describes a microfluidic device that enables pairing and fusion of liposomes. Our device has a dynamic micro array system for pairing liposomes and three-dimensional electrodes for applying voltage for fusing liposomes. We fabricated the dynamic micro array device by standard soft lithography method and integrated three-dimensional (3D) electrode into the device by injecting low melting point alloy. We successfully paired two liposomes in the dynamic micro array device and fused liposomes by the 3D integrated electrode. We believe that this dynamic micro array device will be useful in vesicle fusion and artificial cell studies.