A high throughput electrorotation flow cytometer for single-cell analysis in continuous flows

We report a novel microfluidic-based high-throughput electrorotation flow cytometer for label-free single-cell analysis in continuous flows. This is realized by a heterogeneously integrated microfluidic channel with electrodes for high precision 3D single stream focusing and electrorotation of cells in high speed flows. This device provides 4 orders of magnitude higher throughput than prior electrorotation based cytometers.