A 3D filter for plasma separation from whole blood

This work presented a simple and efficient 3D filter for plasma separation from whole blood. The filter was prepared by an origami of Parylene membrane with different sized micropore arrays and folding grooves. 1.64±0.08 μm micropores were designed for plasma filtration and 39.06±0.39 μm micropores functioned as a by-pass pathway for clogging-free operation. The results showed that the present device could achieve a high yield of 42% for plasma separation from whole blood. Further biological characterization of the separated plasma from the filter was conducted by testing total proteins and blood glucose concentrations, which indicated the present 3D filter separation could achieve a high quality of plasma as the conventional methods.