Centrifuge-based membrane emulsification toward high-throughput generation of monodisperse liposomes

In this paper, we developed a centrifugal emulsification device toward high-throughput generation of monodisperse liposomes. The device generated lipid vesicles in two steps, membrane emulsification by asymmetric through-holes on a plate, and transfer of the emulsions to a planar monolayer at an oil/water interface. As a result of centrifugation of the device in a microtube, we succeeded in collection of cell-sized lipid vesicle. Since a number of the asymmetric through-holes can be fabricated on the plate by standard photolithographic process, and the reservoir for inner solution has relatively large volume (40 μl), the proposed device will be a powerful tool for massive generation of liposomes.