On-chip internalization process of an intracellular nanobot into a single cell

This paper reports on-chip internalization of an intracellular nanobot into a single cell with a microfluidic device. The nanobot was made of a Ni sputtered carbon naocoil and driven by a rotating magnetic field. Here, we fabricated the microfluidic device to capture a single cell by a soft lithography technique and demonstrated on-chip internalization of the nanobot. As a result, a single cell was captured in the microfluidic device and the nanobot was successfully internalized into the trapped cell. Additionally the nanobot could be driven at a speed of 0.35 μm/s inside the cell. This result indicates the potential of the nanobot which can move to any positions inside a single cell for intracellular stimulation.