Microfluidic chip having multi fluorescence microsensors for spatiotemporal sensing of culture environment

We proposed on-chip spatiotemporal sensing for monitoring interactions between cells and environment using fluorescence multi-microsensors. The microfluidic chip integrating fluorescence multi-microsensor is used to construct the harmonious environment like in vivo to reconstruct high-quality organs ex vivo. Fluorescence sensing is suitable for spatiotemporal sensing with high spatial resolution because wiring to many microsensors limits the number of sensors. First, we classify optical sensing method. Fluorescence and polarization sensing are suitable for spatiotemporal sensing such as physiological and mechanical parameters in culture environment. As a demonstration of spatiotemporal sensing of culture environment, Spatiotemporal variation of pH, Ca²⁺ was measured and during transformation of octacalcium phosphate (OCP) to hydroxyl apatite (HA) inside the microfluidic chip using fluorescence multi-microsensors.