Stroboscopic method of fluorescence analises of optically solid media

In the present paper curried out a comparative analysis between the stroboscopic and phase-modulated methods of fluorescence analysis of optically solid media. It is shown that both methods have advantages and disadvantages, but the stroboscopic method is more sensitive. Parameters of fluorescence spectroscopy, like: radiation spectrum, life duration, quantum yield, fluorescence anisotropy - represent the sensitive functions of the processes, which depend of the life duration of excited condition. The molecules can participate in these processes which are in 10 nm distance from fluorofore at the moment of excitation. Therefore, the stroboscopic method has wide application in biology, medicine, material studies and nanotechnology, and of course, in ecology.