The influence of grafting chain length on the temperature response and separation performance of stationary phase with temperature response

Three kinds of chromatography materials with temperature response have been prepared, and they were characterized by IR and TGA. By using the three materials, we studied the separation performance of four different steroids under 5 different temperatures. The results showed that bigger grafting density would lead to shorter chain, lower temperature response and longer retention time. Thus, it is important for the preparation of stationary phase with temperature response ability to choose adaptive grafting ratio, grafting density and grafting chain length.