Hybrid digital holographic microscope for simultaneous measurement of 3D phase and 3D fluorescence distributions and its signal processing

A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and fluorescence images is proposed. The proposed microscopic system is designed by combination of a transmission type off-axis digital holographic configuration and a reflection type Fresnel incoherent holographic configuration, aimed for biological application. Owing to the single acquisition, signal processing which separates a superposed hologram is proposed.