An electroporation system for targeted cellular drug delivery

An electroporation system allowing control of pulse amplitude, frequency, and duration is proposed. The system was implemented using a programmable lock-in amplifier and switch module. A software data acquisition system was designed to allow the user to control the pulse parameters and data acquisition setting. The system was validated by applying 1 V_p-p pulses at 45 kHz to an array of gold electrodes seeded with HCT-116 colorectal cancer cells. Unseeded naked electrodes were used as controls. The experimental results of this study demonstrated a transient response and recover of HCT-116 cells to the applied pulses. Small changes in the naked electrodes were also observed following the electroporation pulses.