Noise suppression with additional reference electrode for time-dependent protein sensing tests with Si nanograting FETs

We propose a method to suppress the electrolyte potential noise in time-dependent protein sensing tests with an additional reference electrode, which doesn't have the challenging requirements as for the reference FET (REFET). The noise is recorded by the additional electrode and then suppressed in the sensing results. This noise is likely due to the electrochemical reaction at the electrolyte - solution gate interface. Results suggest increased readability with reduced signal level variation and increased credibility. The limit of detection (LOD) decreases by 50% ∼ 70%.