Investigating the utility of in vivo bio-impedance spectroscopy for the assessment of post-ischemic myocardial tissue

Increased myocardial structural heterogeneity in response to ischemic injury following myocardial infarction (MI) is purported as the mechanism of ventricular arrhythmogenesis. Current modalities for in vivo assessment of structural heterogeneity for identification of arrhythmogenic substrate are limited due to the complex nature of the structural microenvironment post-MI. We investigated the utility of in vivo bio-impedance spectroscopy (BIS) in a large post-infarct animal model for differentiation between normal and infarcted tissue. We also investigated the quantitative effects of adipose and collagen on BIS assessment of myocardium. The results indicate that the degree of myocardial injury following chronic post-infarction remodeling could be reliably quantified (performed in triplicates) using BIS. Furthermore, the presence of intramyocardial adipose tissue that develops in conjunction with collagen within the infarct zone had a greater and significant influence on BIS then collagen tissue alone. These preliminary results indicate a potential role of BIS for quantitative assessment and characterization of complex arrhythmogenic substrates in ischemic cardiomyopathy.