Microbiosensor for electrical impedance spectroscopic study of melanoma cells

This paper presents the fabrication and characterization of an interdigitated microelectrode array (IDMEA) biosensor. Electrochemical impedance spectroscopy and cyclic voltammetry were used to study the cellular activities of B16 melanoma cell line C57BL, including the kinetics of cell adhesion and spreading on IDMEA. Impedance spectra of B16 melanoma cells on microelectrodes were obtained in cell 0.1 M PBS with 50 mM [Fe(CN)₍₆₎]^(3-/4-) as redox species. Randles equivalent circuits were used to model the electrochemical processes. Impedance spectra allowed us to analyze the changes in the double layer capacitance and charge transfer resistance due to cell attachment on the interdigitated microelectrodes.