Ultrasonic characterization of cell culture medium in the temperature range from 25°C to 50°C

The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.