Expression of antigens in Immunocytochemical images utilizing an image analysis method

Immunocytochemistry is a common laboratory technique in which the quality of interaction between antibody antigen is evaluated on a cell surface. The amount of binding between antibodies and cancerous cells in Immunocytochemical images indicates how much the antibody is effective for treating the cancerous cell. In this paper, an automated system has been proposed for the first time, to measure such binding, utilizing well-known image processing methods. The experiments are simulated using the collected reference images in this research which can be used as a benchmark in the future researches. Proposing this method leads to an accurate and fast evaluation of the antigens' expression along with more reduced human-error factors.