Effects of High Temperature Stress on Transgene Expression and DNA Methylation Pattern

Abstract-Studies about the variation of DNA methylation pattern under high temperature stress (37˚C) contribute to a comprehensive understanding of the apparent regulation of biological functions. Based on the in vitro cultivation of genetically modified birch (Betula platyphylla Suk.) clones for high-temperature stress, the paper detected exogenous gene transcription level differences with RT-PCR. Methylation-sensitive amplified polymorphism (MSAP) analysis the variation of DNA methylation pattern in transgenic birch. In this paper we used ten pairs of primers MSAP to deal with genetically modified DNA screening birch. The study revealed that high temperature stress can lead the level of transcriptional expression of transgene decrease, while endogenous levels of DNA methylation, including the overall methylation, the lateral semi-cytosine methylation as well as the inside of cytosine methylation level of the whole is almost twice than the control.