Purification and Identification of Soluble Catechol-O-Methyltransferase from Rat Liver

Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree. The molecular was 25 kD. Mass spectrometry and mascot were employed for further identification.