Preparation and Structure Analysis of Chondroitin Sulfate from Pig Laryngeal Cartilage

The chondroitin sulfate with high purity was extracted by papain hydrolysis, isolated and purified by tricloroacetic acid, alcohol precipitation, dialysis and DEAE-Sepharose Fast Flow ion exchange column chromatography. The structure was identified by biological enzymolysis and spectral analysis. GC data exhibited that neutral monosaccharide in chondroitin sulfate only included 0.84% of xylose and 1.28% of galactose. IR data showed that chondroitin sulfate included chondroitin-4-sulfate and chondroitin-6-sulfate isomer in a molecular chain. The results of enzyme hydrolysis and spectra analysis, including reverse phase high performance liquid chromatography, mass-spectrum and NMR, indicated that ChS from pig laryngeal cartilage contains the repeated units of GlcA(β1-3)GalNAc4SO₃ and GlcA(β1-3)GalNAc6SO₃. It may not have the structure characteristics of keratan sulfate and dermatan sulfate such as NeuAc (α2-3) Gal (β1-4) GlcNAc4SO₃ units and GlcA2SO₃ (β1-3) GalNAc4SO₃, GlcA2SO₃ (β1-3) GalNAc6SO₃ units. This may explain the internal mechanism of its antioxidation.