Numerical Simulation of the Horizontal Rotating Bioreactor

To simulate the internal pressure numerically for the horizontal rotating bioreactor at 15rpm and to study its effect on the cells culture. Using both the theoretical calculation and the FLUENT 6.0 software to calculate and to simulate respectively the pressure inside the bioreactor, we verified experimentally the calculation result by bone marrow-derived mesenchymal stem cells cultured in the bioreactor. At the rate of 15rpm, the inner pressure of the rotating bioreactor obtained by both methods is basically consistent. It is also found that the dynamic pressure (p_dyn = 125 ρπ²r²) of bioreactor in central area (0 <; r <; 0.28cm) distributed uniformly except near the inner wall area (0.28cm ≤ r ≤ 3.0cm) . The static pressure can be described by the equation p_sta = ρg[R-rCos(ωt+φ)]. Cells are morphology fullness, three-dimensional sense strong, matrix secretion blooming, protuberance and pseudopods rich; pseudopodia are fusion with the edge of materials surface. At the rate of 15 rpm, periodical pressures of bioreactor p = ρg[R-rcos(ωt+φ)] is beneficial for substance transfer between cells and on the other hand a new method for engineering stem cells faster culture is put forward in this paper.