100 picoliter droplet handling using 256 (28) microvalve array with 18 multiplexed control lines

100 picoliter droplet handling with 256 (2⁸) microvalves is demonstrated. The 256 microvalves are positioned on the 256 cross points of 16 vertical fluidic channels and 16 horizontal fluidic channels. 256 microvalves are individually controlled by multiplexed pneumatic circuit having only 18 (2^*8+2) pneumatic control lines. The 100 picoliter order droplet of sample is handled in inert liquid to avoid evaporation. The droplets about 100 pl are successfully transferred to any directions by 256 microvalves.