Dynamic Monitoring of PEG-Mediated Cell Fusion by RT-CES System

The response of MCF7 and A549 cells to the PEG treatment was monitored in the terms of impedance-related cell index curves by RT-CES system. The treatment of 50% PEG to MCF7 and A549 cells led to the one-stage drop of cell index with the culture time. Longer duration of PEG action to cells shortened the net detachment duration of cells from the surface of the electrode. The treatment of 15% PEG to MCF cells resulted in two-stage drop of cell index. These were attributed to PEG-mediated cell fusion, which was supported by SEM results.