Chemical stimulation of Aplysia californica ganglion with microfluidic signal generators

We implemented a microfluidic signal generator chip for the in-vitro study of the nervous system of Aplysia californica under localized chemical stimulation. The PDMS chip implements a one-bit pulse-code modulator that digitally controls the concentration of the non-hydrolysable cholinergic agonist carbachol injected directly above a ganglion. The chip was successful in inducing a burst of ingestive patterns.