A Novel Auto Focusing Algorithm for a Micromanipulation System

With rapid development of biotechnology and other relevant technology, micromanipulation in biotechnology has been drawing the attention of scholars home and abroad. Especially micromanipulation on micro scale is demanded, however, most of the current commercial micromanipulation systems adopt manual operation mode by mechanical or hydraulic transmission. In this circumstance, we develop and study a computer-controlled system. The system includes visualization part, human interface part and micromanipulator part. It can be used for cell puncturing and many other micromanipulations. In this paper we proposed a novel auto focusing algorithm. The whole visualization part includes two parts: auto locating part and auto focusing part. It not only can realize real-time monitoring, but also can accurately locate the target's position by visualization part and human interface part. The auto focusing algorithm has high correct ratio and high precision and stability.