Effect of magnetic fields on the enzymatic activity of plasmin

The authors investigated the effect of magnetic fields on the enzymatic activity of plasmin concerning the dissolution of thrombus. Plasmin activity in magnetic fields up to 8 T was assessed using a synthetic substrate for plasmin, D-Val-Leu-Lys-pNA. After 40 min or more incubation, absorbance of the mixture exposed to an 8 T magnetic field at 37/spl deg/C was 7-11% lower than control sample. The results show that the enzymatic activity of plasmin was affected by a magnetic field at 8 T. This suggests that magnetic fields have an effect on the active center of the enzymatic reaction of plasmin with a substrate, especially on the hydrogen bonds of the charge-relay system.<>