Spatial Transepithelial Electrical Resistance Profiling with Multi-Electrode Array for Nondestructive Cell Culture Model Monitoring

We present a spatial probing system for 2- dimentional transepithelial electrical resistance (TEER) profiling using a multi-electrode array. This approach allows for precise monitoring of local changes in electrical resistance across the cell culture models without disruption. The prototype sensor system generated spatial TEER maps, which were compared to immunostaining images. The proof-of- concept results showed a strong correlation between the spatial TEER data and the immunocytochemistry results, validating the accuracy and reliability in nondestructive cell culture model evaluation. The system's capability for continuous monitoring over more than 50 days was also demonstrated. The proposed system also holds a significant potential for cell culture model development and manufacturing, as long-term and detailed evaluation is critical for quality control in biological and pharmaceutical research.