Automated Optical Tweezers for Enhanced Bioparticle Analysis via Combined Scattering and Raman Spectroscopy

This paper describes the development of an optical tweezers system that operates in fully automatic mode. It features image recognition for particle tracking, allowing for the optical trapping and analysis of identified targets. The system can per-form analysis of forward scattered light and Raman spectroscopy of the trapped particles, facilitating the automated analysis of a large number of samples without manual intervention. By leveraging combined analytical methods and AI for robust classification, this system contributes to the advancement of automated diagnostic tools. Preliminary results demonstrate the system's effectiveness using different kinds of standard and biofunctionalized PMMA microparticles.