Multichannel Electrophysiological Recording With Spike Detection and Sorting in a Duty-Cycled Wireless Optogenetic Headstage

Neural signal recording and optical stimulation using implantable devices have become a ubiquitous method to treat brain disorders, yet there lie some shortcomings, such as size, weight, and functionalities of the implants. This work presents a commercial off-the-shelf (COTS) component-based miniaturized wireless optogenetic headstage with simultaneous optical stimulation and electrophysiological recording for freely moving rats. The system includes a battery-based neural stimulator consisting of a low-dropout (LDO) regulator, an oscillator, and a
$\mu $
LED. The electrophysiological signal recording system includes an intracortical neural probe implemented on a shape memory polymer (SMP) substrate, an array of neural amplifiers with an integrated analog-to-digital converter (ADC), a transceiver IC, and a ceramic antenna. A digital sub-1-GHz transceiver integrated with a low-power microcontroller (MCU) is used to transmit the acquired neural data to a remote receiver unit, followed by offline spike detection and sorting in LabVIEW. The front-end recording amplifiers provide a gain of 45.7 dB with the input-referred noise of
$2.4 \mu \text {V}_{\text {rms}}$
. The integrated multiplexer (MUX) with the ADC allows sampling of the amplified voltage at a configurable sampling rate of 160–480 kSamples/s. The total power consumption of the stimulation and the recording system is 23 mW. The dimension of the headstage device is
$13.5\times21.3$
mm, weighing 4 g without the battery. The system is experimentally validated in an in vivo setting by placing the headstage on the head of a male rat and recording the neural signals from the ventral tegmental area (VTA) of the brain. This integrative neural signal recording and spike sorting approach would be useful for the development of a closed-loop neuromodulation system.