UV Spectral Characterization and Quantitative Study of Ethanol in Ethanol/Water Solutions Using Spectrophotometry for Standardization Applications

Ethanol/Water aqueous solution as a reference material (RM) is of great importance for many applications and disciplines, e.g. food industry. The present work describes the spectroscopic quantitative study of solutions of ethanol in water in five different concentrations. Concentration range from 600 ppm to 8000 ppm has been studied and shown excellent fulfilment of Beer’s Lambert Law. The concentration measurement method comprises the use of a simple gravimetric preparation method of standard solutions, as well as using a high-accuracy spectrometric system. Using the high-performance spectrophotometer, the results show good repeatability, reproducibility, and relatively low associated uncertainty in concentration measurements, which can be considered a great advantage toward producing new reference material of Ethanol/Water solutions with different concentrations following the main guidelines of the relevant standards to produce a certified reference material (CRM) as per international standards such as ISO/IEC 17034, and ISO Guides 30, 31, 33, and 35.