Rapid detection of fibrinogen and fibrin degradation products by latex piezoelectric immunoassay

It was developed a conventional immunosensor for fibrinogen and fibrin degradation products (FDP) to combine a quartz crystal microbalance (QCM) with the agglutination reaction of immunized latex beads. We successfully measured FDP concentration of in human serum within 10 min by QCM method. The detection range of QCM immunosensor is covered with screening concentration of FDP in serum (less than 10 /spl mu/g/ml of FDP). The time course of latex agglutination obtained from QCM immunosensor is synchronized to that of latex photometric immunoassay. Frequency shift on immunoreaction explains the increased adsorption amount of agglutinated latex on QCM.