Development and Scientific Application of Deep-sea Multiple in situ nucleic acid collections (MISNAC) apparatus

The world of marine microbes is still mysterious to us. The study of microbial community structure is limited by sampling analysis tools. Nowadays, popular methods for retrieving microbes are still limited. And the quality of the obtained samples cannot meet higher demands of scientific research. This paper presents an apparatus used for deep-sea in situ nucleic acid collection, which is specially developed to improve samples quality. It is designed for in situ filtration, cell lysis, and nucleic acid collection of deep-sea microbes. It has been fully verified that the apparatus has excellent continuous sampling capability. In recent scientific deployment, many achievements have been obtained. The obtained samples would reveal the changing rules of the microbial community structure in deep sea at different times. By designing multiple in situ nucleic acid collections (MISNAC) apparatus, we aim to provide a new research tool to promote the study of marine microbiology.