Multi-modal Imaging in Live Cell Microscopy

Methods of high resolution deep view microscopy of living cells and tissues are reported. These techniques include Structured Illumination Microscopy (SIM) for optimizing resolution, Light Sheet Fluorescence Microscopy (LSFM) for 3D imaging at low light exposure and a combination of both methods. Applications are concentrated on localization of cellular metabolites, signal transduction as well as cell and tissue diagnostics.