Optical Fiber SPR Sensor for Highly-Sensitive Detection of Cholesterol Concentration

A reflective surface plasmon resonance (SPR) cholesterol sensor based on cholesterol oxidase was proposed. Due to the enzymatic reaction between cholesterol and cholesterol oxidase, the refractive index of the solution near the sensor area change, which will then induce the wavelength shift of the SPR spectrum. Experimental results showed that in the concentration range of 0-500nM, the SPR spectrum wavelength shifts 27.691nm. In the concentration range of 0-30nM and 50-500nM, the measurement sensitivities of the SPR sensor could reach 0. 39nm/nM and 12.34nm/lg[M] respectively. In addition, the sensor behaves small in size and good stability, which has great potential application in biomedicine and human health monitoring.