3D Printed Microfluidic Actuation System for Multi-step Paper-based Assays

We present a novel 3D printed Microfluidic Actuation System for lateral flow assay in low resource settings. The system is used to deliver reagents for multi-step assays from blisters placed into cavities in the 3D printed assembly. The system is operated by manually depressing the blister housing and rotating to the next blister location. This is repeated for each step in the assay to enable a simple and repeatable method of delivering specified volumes to the assay at arbitrary time intervals as required by the assay. The blisters provide for robust storage while maintaining consistent aliquots for the assay. We characterize the percent of the total volume delivered to the lateral flow assay from the blisters including the volume dispensed at given time intervals.