Continuous Glucose Monitoring of 3D Tissue Using a Perfusable Device

We developed a continuous glucose monitoring (CGM) system for the monitoring of 3D tissue in vitro using a perfusable device. The system consists of perfusable cell culture unit and CGM unit that has a fluorescent-based glucose responsive hydrogel sensor, LED and a photodiode on a chip; the unit is placed under a fluidic channel. This system enables in situ observation of the glucose concentration in culture medium to control the environment of the culture systems. We confirmed that the fluorescence intensity increased depending on the glucose concentration; ranging from 0 mg/dL to 1,000 mg/dL. We successfully cultured the 3D tissue in the perfusable unit and monitored the glucose concentration of medium for 24 hours.