Multiple-compartment chip for parallel recordings of epileptic activity from organotypic cultures

Organotypic hippocampal slice culture is an in vitro model of post-traumatic epilepsy. To increase experimental throughput for development of anti-epileptic drugs, we designed a system for parallel recordings of neural activity from several cultures. We recorded spontaneous electrical activity from four organotypic cultures on the same recording chip for more than seven weeks. We confirmed that hippocampal cultures develop spontaneous epileptiform activity. Cultures exhibited a quiet latent period followed by interictal and ictal epileptiform activity.