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Nanobiotechnology, IET

Issue 4 • Date December 2013

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Displaying Results 1 - 4 of 4
  • Nanoscale electrode arrays produced with microscale lithographic techniques for use in biomedical sensing applications

    Page(s): 125 - 134
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (630 KB)  

    A novel technique for the production of nanoscale electrode arrays that uses standard microfabrication processes and micron-scale photolithography is reported here in detail. These microsquare nanoband edge electrode (MNEE) arrays have been fabricated with highly reproducible control of the key array dimensions, including the size and pitch of the individual elements and, most importantly, the width of the nanoband electrodes. The definition of lateral features to nanoscale dimensions typically requires expensive patterning techniques that are complex and low-throughput. However, the fabrication methodology used here relies on the fact that vertical dimensions (i.e. layer thicknesses) have long been manufacturable at the nanoscale using thin film deposition techniques that are well established in mainstream microelectronics. The authors report for the first time two aspects that highlight the particular suitability of these MNEE array systems for probe monolayer biosensing. The first is simulation, which shows the enhanced sensitivity to the redox reaction of the solution redox couple. The second is the enhancement of probe film functionalisation observed for the probe film model molecule, 6-mercapto-1-hexanol compared with microsquare electrodes. Such surface modification for specific probe layer biosensing and detection is of significance for a wide range of biomedical and other sensing and analytical applications. View full abstract»

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  • Production of nano zinc, zinc sulphide and nanocomplex of magnetite zinc oxide by Brevundimonas diminuta and pseudomonas stutzeri

    Page(s): 135 - 139
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (372 KB)  

    ZnO (Zincite) nanoparticle has many industrial applications and is mostly produced by chemical reactions, usually prepared by decomposition of zinc acetate or hot-injection and heating-up method. Synthesis of semi-conductor nanoparticles such as ZnS (Sphalerite) by ultrasonic was previously reported. In this work, high-zinc tolerant bacteria were isolated and used for nano zinc production. Among all isolated microorganisms, a gram negative bacterium which was identified as Brevundimonas diminuta could construct nano magnetite zinc oxide on bacterial surface with 22 nm in size and nano zinc with 48.29 nm in size. A piece of zinc metal was immersed in medium containing of pure culture of B. diminuta. Subsequently, a yellow-white biofilm was formed which was collected from the surface of zinc. It was dried at room temperature. The isolated biofilm was analysed by X-ray diffractometer. Interestingly, the yield of these particles was higher in the light, with pH 7 at 23°C. To the best of the authors knowledge, this is the first report about the production of nano zinc metal and nano zinc oxide that are stable and have anti-bacterial activities with magnetite property. Also ZnS (sized 12 nm) produced by Pseudomonas stutzeri, was studied by photoluminescence and fluorescent microscope. View full abstract»

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  • Design and simulation of perturbed onion-like quantum-dot-quantum-well (CdSe/ZnS/CdSe/ZnS) and its influence on fluorescence resonance energy transfer mechanism

    Page(s): 140 - 150
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (869 KB)  

    In this study, the authors investigate one of the biological sensory applications (fluorescence resonance energy transfer (FRET)) that has astonishing influence on implementation of the bio-medical assays. For the first time, in this study, the new inorganic modified nanoparticle structure (quantum-dot quantum-well (QDQW) heteronanocrystal) is used as donor particle. By considering the mentioned structure, the authors can easily manipulate the donor emission spectrum and all parameters of FRET, such as overlapping between the donor emission and acceptor absorption. In this procedure, the F??rster radius can be completely controlled. Moreover, the quantum confinement effects, including the strong-confinement and the weakconfinement regime, are investigated for FRET essential variables. As a main goal of this study, the authors will mathematically explore the influences of perturbation on the intrinsic parameters of nanoparticles (lifetime and bandwidth of emission spectrum) and on the sensitivity of FRET. It can be revealed that the presence of perturbation in nanoparticle has a devastating influence on biological assay characteristics, which will be probe in presented simulations. Furthermore, the physically analysing of QDQW heteronanocrystal structure is performed by the accurate eight-band K.P theory and finiteelement method. View full abstract»

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  • Effect of gold nanoparticles on photodynamic efficiency of 5-aminolevolenic acid photosensitiser in epidermal carcinoma cell line: an in vitro study

    Page(s): 151 - 156
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (700 KB)  

    In the recent years, enhanced functionality of treatment systems based on nanostructures has attracted a lot of interest. Photodynamic therapy (PDT) is one such treatment method. Here, the authors report the results of the investigations on synthesis and characterisation of gold nanoparticles (GNPs) and their application in PDT along with 5-aminolevolenic acid (5-ALA) (as photosensitiser) with no conjugation. Three sizes of GNPs were synthesised and their cytotoxicity was investigated by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on epidermal carcinoma cell line. The results showed that the PDT efficiency of ALA increased in presence of GNPs. This effect was more considerable for 4 nm particles. View full abstract»

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Aims & Scope

IET Nanobiotechnology covers all aspects of research and emerging technologies including fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale.

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