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Nanobiotechnology, IET

Issue 3 • Date September 2012

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Displaying Results 1 - 6 of 6
  • Modelling of internal architecture of kinesin nanomotor as a machine language

    Page(s): 87 - 92
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (463 KB)  

    Kinesin is a protein-based natural nanomotor that transports molecular cargoes within cells by walking along microtubules. Kinesin nanomotor is considered as a bio-nanoagent which is able to sense the cell through its sensors (i.e. its heads and tail), make the decision internally and perform actions on the cell through its actuator (i.e. its motor domain). The study maps the agent-based architectural model of internal decision-making process of kinesin nanomotor to a machine language using an automata algorithm. The applied automata algorithm receives the internal agent-based architectural model of kinesin nanomotor as a deterministic finite automaton (DFA) model and generates a regular machine language. The generated regular machine language was acceptable by the architectural DFA model of the nanomotor and also in good agreement with its natural behaviour. The internal agent-based architectural model of kinesin nanomotor indicates the degree of autonomy and intelligence of the nanomotor interactions with its cell. Thus, our developed regular machine language can model the degree of autonomy and intelligence of kinesin nanomotor interactions with its cell as a language. Modelling of internal architectures of autonomous and intelligent bio-nanosystems as machine languages can lay the foundation towards the concept of bio-nanoswarms and next phases of the bio-nanorobotic systems development. View full abstract»

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  • Macroporous/mesoporous bioglasses doped with Ag/TiO2 for dual drug action property and bone repair

    Page(s): 93 - 101
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (1104 KB)  

    A series of macroporous-mesoporous bioglasses (MMBGs) doped with Ag, TiO2 and Ag/TiO2 have been successfully prepared using the nature plants as the macroporous templates and P123 as the mesoporous one. The dual drug action (antifungus and anti-inflammatory) and bone regeneration systems have been obtained by Ibuprofen (Ibu) loaded. Meanwhile, the antifungus as well as drug control release profiles of these materials have been investigated. Compared with pure MMBGs, MMBGs-Ag, MMBGs-TiO2 and MMBGs-Ag/TiO2 showed much higher antimicrobial efficiency, faster hydroxyapatite-forming ability and better drug sustained release performance. The multifunctional hierarchical porous bioglasses materials may have potential applications in bone tissue engineering. View full abstract»

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  • Immobilisation of cobaltferritin onto gold electrode based on self-assembled monolayers

    Page(s): 102 - 109
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (499 KB)  

    The iron storage protein, ferritin, has a cavity of ~7~nm in diameter in which iron is oxidised and stored as a hydrated oxide core. Electron transfer is known to be an important step in the sequestering of iron by cellular ferritin. The cavity was used as a nanocontainer to grow cobalt nanoparticles. The immobilisation of ferritin on the electrode surface is essential for various bioelectronic applications. A cobaltferritin-immobilised electrode based on self-assembled monolayer (SAM)-modified gold electrode was developed. The cobaltferritin-immobilised SAM-modified electrode was characterised by electrochemical and atomic force microscopy (AFM) techniques. The results indicated that cobaltferritin was selectively immobilised onto succinimidyl alkanedisulfide-modified Au electrode by the covalent interaction between cobaltferritin and the terminal functional groups of the SAMs. The cobaltferritin immobilised modified electrode showed a direct electron transfer reaction between cobaltferritin and the electrode. The electrochemically regulated uptake and release of cobalts for cobaltferritin immobilised on the SAMs were demonstrated. The results obtained in this study indicate that cobaltferritin has potential for a biomaterial in nanoscale synthesis for potential magnetic, catalytic and biomedical-sensing applications. View full abstract»

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  • Effect of biologically synthesised silver nanoparticles on Staphylococcus aureus biofilm quenching and prevention of biofilm formation

    Page(s): 110 - 114
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (370 KB)  

    The development of green experimental processes for the synthesis of nanoparticles is a need in the field of nanotechnology. In the present study, the authors reported rapid synthesis of silver nanoparticles using fresh leaves extract of Cymbopogan citratus (lemongrass) with increased stability. The synthesised silver nanoparticles were found to be stable for several months. UV-visible spectrophotometric analysis was carried out to assess the synthesis of silver nanoparticles. The synthesised silver nanoparticles were further characterised by using nanoparticle tracking analyser (NTA), transmission electron microscope (TEM) and energy-dispersive x-ray spectra (EDX). The NTA results showed that the mean size was found to be 32-nm. Silver nanoparticles with controlled size and shape were observed under TEM micrograph. The EDX of the nanoparticles confirmed the presence of elemental silver. These silver nanoparticles showed enhanced quorum quenching activity against Staphylococcus aureus biofilm and prevention of biofilm formation which can be seen under inverted microscope (40X). In the near future, silver nanoparticles synthesised using green methods may be used in the treatment of infections caused by a highly antibiotic resistant biofilm. View full abstract»

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  • Dynamic analysis of fixed-free single-walled carbon nanotube-based bio-sensors because of various viruses

    Page(s): 115 - 121
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (508 KB)  

    In the present study, the vibrations of the fixed-free single-walled carbon nanotube (SWCNT) with attached bacterium/virus on the tip have been investigated. To explore the suitability of the SWCNT as a bacterium/virus detector device, first the various types of virus have been taken for the study and then the resonant frequencies of fixed-free SWCNT with attachment of those viruses have been simulated. These resonant frequencies are compared with the published analytical data, and it is shown that the finite element method (FEM) simulation results are in good agreement with the analytical data. The results showed the sensitivity and suitability of the SWCNT having different length and different masses (attached at the tip SWCNT) to identify the bacterium or virus. View full abstract»

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  • Enhanced gene transfer with multilayered polyplexes assembled with layer-by-layer technique

    Page(s): 122 - 128
    Save to Project icon | Click to expandQuick Abstract | PDF file iconPDF (476 KB)  

    Successful gene therapy asks for multifunctional vectors which can not only protect DNA from degradation but also transfer it into nuclear and subsequently express the loaded gene. Here we reported a novel multilayered delivery system constructed with DNA, protamine (Pro) and polyethylenimine (PEI) via lay-by-layer (LbL) technique, which posed multifunctions. DNA was previously condensed into a compact core with Pro which also contained nuclear localisation signals (NLS) domains for nuclear transfer. Then additional DNA was deposited as the first layer onto the cationic core via the electrostatic attraction which would increase the loading dose of DNA. At last, PEI was absorbed as the outmost layer to achieve the endosomal escape. Therefore a quaternary polyplexes which offered high loading of DNA, nuclear transfer ability and endosomal escape capability was constructed with the LbL technique. The obtained quaternary polyplexes showed positive surface charge, spherical morphology, a relatively narrow particle size distribution and strong DNA protection capability. Compared with commercially available PEI/DNA complexes, the novel multifuctional vector exhibited not only lower cytotoxicity (P<;0.05) but also higher transfection efficiency in HepG2 and HeLa cells (P<;0.05) in vitro test. View full abstract»

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Aims & Scope

IET Nanobiotechnology covers all aspects of research and emerging technologies including fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale.

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