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Human Health and Biomedical Engineering (HHBE), 2011 International Conference on

Date 19-22 Aug. 2011

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Displaying Results 1 - 25 of 350
  • [Front cover]

    Page(s): c1
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  • [Title page]

    Page(s): 1
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  • [Copyright notice]

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  • Table of contents

    Page(s): i - xxii
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  • Genetic analysis on viable but non-culturable Salmonella pullorum by mRNA differential display

    Page(s): 1 - 6
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    Salmonella pullorum was induced using liquid lysogeny broth medium at 4°C into viable but non-culturable (VBNC) state. The activated VBNC genes were screened by mRNA differential display. VBNC bacteria transformed to the spherical-rods or spherical shape and aggregated. Sequence analysis showed two VBNC differential fragments respectively belonged to partial sequences of ATP-dependent RNA unwindase rh1B gene and tRNA 2-selenouridine synthase ybbB gene in Salmonella pullorum. The former sequence may be the structure domain of ATPase responsible for coding DEAD-box unwindase, and the latter may catalyze 2-uridine converting into 2-selenium uridine in tRNA molecules, responsible for participation in the codon-anticodon interaction and the amino acylation of tRNA. View full abstract»

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  • Rapid determination of lignans in Schisandra Chinensis and Schisandra sphenanthera by micellar electrokinetic capillary chromatography

    Page(s): 7 - 10
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    A simple and rapid micellar electrokinetic capillary chromatography (MEKC) method has been developed for the simultaneous determination of six lignans, schizandrin, Gomisin A, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C, in fruits of S. chinensis and S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained, in the buffer of 10 mM phosphate-37.5 mM SDS-35% v/v acetonitrile (pH 8.0) at the separation voltage of 28.0 KV and detection at 214 nm, the plant samples could be detected within 8.2 min. Analysis yielded good reproducibility (RSD between 1.19-2.28%) and good recovery (between 92.2-102.9%). The method is quick, sensitive, accurate and it could be applied to the quality control of S. chinensis and S. sphenanthera. View full abstract»

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  • Immunomodulatory effect of lectin from Musca domestic pupa on immunosuppressive mice: Effect of lectin on immunosuppressive mice

    Page(s): 11 - 14
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    The present study was conducted to elucidate the immunomodulatory effect of a lectin from Musca domestic pupa (MPL) in vivo. The effects of MPL (200, 400 and 800 μg/kg body wt.) on immune organ weight, mitogen-induced proliferation of spleen lymphocyte, phagocytosis activity of macrophages, NK cell activity, and the mRNA expression of immune factor in spleen like interleukin (IL) and interferon (IFN) were investigated in cyclophosphamide (CP) immunosuppressive mice. In comparison with that of immunosuppressive mice, the weight index of spleen and thymus were significantly increased (p<;0.05), the proliferation of spleen lymphocyte was enhanced, NK cell activity and phagocytosis activity of macrophages had been improved (p<;0.01) and the mRNA expression of IL-6, IL-12 and INF-γ in spleen were potentialized in a dose-dependent manner by MPL administration. These results demonstrated that MPL has a remarkable immunomodulatory effect on immunosuppressive mice, which suggested that MPL could be useful for further study as an immunomodulating agent. View full abstract»

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  • Culture and identification of dendritic cells from mouse bone marrow in vitro

    Page(s): 15 - 18
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    Establish a method of cultivation and purification of dendritic cells (DCs) from mouse bone marrow in vitro to provide experimental material and establish basis for the study of immunological tolerance. BALB/c (H-2d) mice spleen lymphocytes and bone marrow cells were extracted under sterile conditions and cultured in RPMI 1640 medium. DCs were induced by recombinant granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin (IL-4). Then, the morphology of DCs was observed under light microscopy and CD80, CD86 expression levels were detected by flow cytometry. The results showed that bone marrow cells appeared irregular and formed dendritic processes after 3 days cultured with stimulating factors in vitro which present the typical morphology of dendritic cells; Costimulatory molecules (CD80, CD86) were low expression on the cellular surface. Obtaining DCs from bone marrow cells is a promising method, which might meet further study on function, marker and anti-tumor role of DCs in vitro. View full abstract»

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  • Application of CD4+ CD25+ T regulatory cells in trachea allograft transplant model of mice to reject anti-graft response

    Page(s): 19 - 23
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    To study the role of CD4+ CD25+ Treg cells in anti-graft rejection response and potential value in application of lung transplatation, we established mice trachea allograft transplant model and sort CD4+ CD25+ Treg cells from mice spleen lymphocytes by MACS. FCM assays were done to analyze the ratio of marker protein, Foxp3, expression in CD4+ CD25+ Treg cells. Alveolar cells were used as a non-specific antigen to activate iDCs into mDCs, and cocultureed with CD4+ CD25+ Treg cells to activate Treg cells. The activated Treg cells were inject into mice with trachea allograft transplantation to reduce rejection response. The allografts were extracted and done pathological examination with H&E staining. The purity of CD4+ CD25+ Treg cells were over 80% and specifically express Foxp3, the organizational structure of implanted trachea showed that the tracheal structure was intact. Our research imply that CD4+ CD25+ Treg cells play an essential role in reducing rejection, and lay the foundation for reject anti-graft response in clinical application. View full abstract»

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  • Rapid and simultaneous determination of rutin, chlorogenic acid and quercetin in mulberry folium leaf by capillary electrophoresis

    Page(s): 24 - 27
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    To improve a capillary electrophoresis method used for the rapid and simultaneous separation and determination of rutin, chlorogenic acid and quercetin. The capillary column without coating (50μm×56cm, the used length: 47.5cm) was used, the solution of 12mM sodium tetraborate pentahydrate (pH 9.0) containing 15% methanol was taken as the running buffer and the temperature was 25 _. The linear relationship, recover rate and repeatability of rutin, chlorogenic acid and quercetin were better when they were detected at wave length 350nm. The method we used should be accurate and suitable for the quality control of mulberry folium leaf which is a crude drug. View full abstract»

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  • The association between dibutyl phthalate (DBP)-induced testicular function damage and endocrine disorders

    Page(s): 28 - 31
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    To explore the association between dibutyl phthalate (DBP)-induced testicular function damage and endocrine disorders by using adult male rats as animal models. 40 healthy male SD rats were randomly divided into three exposure groups (DBP: 1000mg. kg-1. d-1, 500mg. kg-1. d-1, 50mg. kg-1. d-1) and one negative control group (corn oil by gavage: 10mL.kg.d-1), and were killed by cervical dislocation after 5 weeks. Sperm quality, testicular histopathology, oxidative stress and reproductive hormone levels of adult male rats were measured were analyzed. The results showed that sperm concentration and sperm motility decreased (P <;0.05) by the level of exposure groups, malformation rate was significantly increased (P <;0.05) in the exposure groups; SOD, GSH-Px and T-AOC in high and middle dose group were lower (P <;0.05) than those in the control group; MDA values in exposure groups were higher (P <;0.05) than that of the control group; FSH and LH levels increased, and T level decreased (P <; 0.05) by DBP concentration. DBP-induced tissue damage of rat testis was associated with the blood-testis barrier damage, which resulted in the increase of hormone FSH, LH levels, and decrease of T levels. These changes have an effect on the function of seminiferous epithelium, leading to the decrease of sperm counts and sperm motility, and increased of abnormal sperm. View full abstract»

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  • Quantitative determination of total alkaloid in Corydalis Ochotensis Turcz by colorimetry

    Page(s): 32 - 34
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    A quantitative determination of the total alkaloid in Corydalis Ochotensis Turcz was established by acid dye colorimetry. The effects of wavelength, pH of the phosphate buffer and the quantity of acid dye were studied. Two different dyes were compared according to the yield of berberine hydrochloride. The method with bromophenol blue dye was proved to be more accurate, more stable with good reproducibility in testing experiments. So, it was fit for quantitative determination of total alkaloid in Corydalis Ochotensis Turcz. View full abstract»

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  • Effect of Prunus Tomentosa Thumb Total Flavone on hair growth

    Page(s): 35 - 38
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    The preliminary laboratory study found that Prunus Tomentosa Thumb Total Flavone (PTTTF) had certain protection function on skin tissues and its subsidiary organs. Combined with civilian application experience, the study adopted C57BL 6 mouse phalacrosis models, the models were randomly divided into 5 groups with 10 in each group, namely: saline group (1 time / day), 70% ethanol group (1 time / day), PTTTF group I (1% PTTTF tincture, 1 time / day), 5% minoxidil tincture group (1 time / day) and PTTTF group II (1% PTTTF tincture, 2 times / day), with the PTTTF was used in external local parts continuously, the length mean of new hair was measured, meanwhile, the tissues of the phalacrosis zones were selected for pathological analysis. The results showed that PTTTF can prominently prolong the hair growth cycle, promote hair growth and promote follicle maturation. Tip: PTTTF has stronger role in promoting hair growth. View full abstract»

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  • A recombinant single-chain trispecific antibody against three toxins and activity in the synchronization detection

    Page(s): 39 - 43
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    The recombinant single-chain trispecific antibody against three toxins including Okadaic acid (OA), saxitoxin (STX) and tetrodotoxin (TTX) was developed. Three single-chain variable fragment (ScFv) genes were linked via linkers (Gly4-Ser)3 to construct fusion ScFv gene OST-ScFv through splicing by overlapping extension. Fusion gene OST-ScFv was 2 256 bp, encoding 752 amino acids, and was inserted into expression vector pET-22b. Recombinant plasmid OST-ScFv-22b was expressed at 37°C and at 25°C by induction with β-D-1-thiogalactopyranoside. The expressed proteins were identified by SDS-PAGE and purified by urea dissolving, gradient dialyzing and ultrafiltrating. Molecular weight of expressed protein was about 79.000 kDa. Competitive indirect enzyme linked immunosorbent assay indicated that the single-chain trispecific antibody which had different combining activity with three toxins was obtained. This work confirmed that multi-specific ScFv antibody could be obtained via linking expression of ScFvs and might also have an application in synchronization detection of toxins. View full abstract»

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  • Construction of expression vector for lvgA/ctxB-EGFP fusion gene and study on its immunogenicity

    Page(s): 44 - 48
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    Legionella pneumophila is an intracellular bacteria, and its successful parasitism in host cells involves two reciprocal phases: transmission and intracellular replication. In this study, we try to construct expression vectors of pcDNA3.1 (+)-lvgA/ctxB-EGFP fused gene and to investigate its transient expressions in NIH3T3 cells. Then preliminary analysis the immunogenicity of lvgA/ctxB fusion gene. The recombinant plamids pcDNA3.1(+)-EGFP, pcDNA3.1(+)-lvgA-EGFP, pcDNA3.1 (+)-ctxB-EGFP, pcDNA3.1(+)-lvgA/ ctxB-EGFP were transfected into NIH3T3 cells respectively with Lipofection method; Transient products were detected by immunofluorescence assay (IFA) and Western blotting. Study on there immunogenicity through the detection of cytokines in mice immunization. Mice were immunized intramusculary with different plasmids DNA as vaccines for immunization by detection the production of IFN-γ, IL-8. It was found that there was green fluorescence on the cells membrane and inside the cells and showed that NIH3T3 cells were transfected by fused expression vectors successfully, the production of IFN-γ, IL-8 by blood of mice immunized with fused genes all higher than pcDNA3.1(+) group and the production of pcDNA3.1(+)-lvgA/ctxB immunized was the most highest. The fused expression vectors were successfully constructed and transfected in NIH3T3 and induced specific immune responses in mice, the group immunized by the pcDNA3.1(+)-lvgA/ctxB was most obviously, which provide the basis for the research on DNA vaccine of Legionella pneumophila. View full abstract»

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  • Design, activity determination and 99mTc labeling of cyclic RGD dimer for targeting integrin αVβ3

    Page(s): 49 - 53
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    This paper was to design a cyclic RGD (Arg-Gly-Asp) peptide tumor inhibitor with high affinity to integrin αvβ3 receptor by molecular docking technique, determinate the characteristics of the cRGD dimer in vitro and prepare 99mTc-cRGD dimer tumor probe. cRGD molecule library was built and an optimal structure of cRGD peptide with the best score that was Cys-Arg-Gly-Asp-(D)Ser-Cys was screened out using DOCK procedure of V-life software. A dimer containing two cRGD peptides linked by Tyr-(D) Ser-Lys-(D)Ser-Ser and with a side chain Gly-Gly-(D)Ala-Gly on lysine residue was synthesisized and 99mTc-cRGD dimer was prepared. The radiolabeled efficiency, stability, water-soluble and affinity of cRGD dimer in vitro were evaluated. Under the reaction condition of room temperature, 1g/L SnCl2·2H2O and the 30 min of reaction time, 99mTc labeling efficiency reached (87.42±3.21) % With Sephadex G10 purification, the radiochemical purity was no less than 95%. In both mediums of saline and fresh human serum, the radiochemical purity kept high stability at room temperature and 37 °C. The equilibrium dissociation constant (Kd) to U87MG human glioma cells was determined and the octanol-water partition coefficient was mearsured. The Kd value is (3.867+0.052)×10-9 mol/L and octanol-water partition coefficient log P value is -1.96±0.01. This in vitro study provides evidence that cRGD peptide screened by computer-aided drug design (CADD) system can bind to integrin αvβ3 with high affinity, and may be a potential candidate radiotracer for integrin αvβ3-positive tumors imaging. View full abstract»

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  • Research for ultramicrofingerprint of Semen Cuscutae

    Page(s): 54 - 57
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    The paper reports an identification method of Semen Cuscutae. The ultramicofingerprint features of Semen Cuscutae is probed with scanning electron microscope and other two different methods, which researched on standard sample and random samples. There is an obvious difference between Semen Cuscutae and its garble by scanning electroscopic according to our research. This method can be used for identification of Semen Cuscutae conveniently, quickly and accurately. View full abstract»

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  • Experimental study on the mechanism of raspberry ketone on simple obesity and insulin resistance relevant to obesity

    Page(s): 58 - 61
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    The Purpose of this research is to discuss the function mechanism of raspberry ketone on simple obesity and the influence of resistance on obesity relevant insulin. There were 40 SD rats of SPF grade, and the male to female ratio was 1:1. They were randomly divided into 5 groups: NC fed with normal diet. MC fed with high-fat diet. RKL, RKM, and RKH groups fed with high-fat diet and were administered intragastrically with 0.5%, 1%, and 2% of RK for 4 weeks. After 8 weeks, some parameters were tested. The results were processed by statistical analysis. Compared with NC, TG, TC, LDL-C, GLU, INS, IRI, FFA, LEP, TNF-α of MC were significantly increased. Therefore, HDL-C, ISI and APN were significantly decreased. Compared with MC, each parameter in RKL, RKM, and RKH was significantly improved. The results showed that RK have anti-obesity function through combined effects of improving insulin resistance. View full abstract»

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  • Determination of raspberry ketone in raspberry by high performance liquid chromatography tandem mass spectrometry

    Page(s): 62 - 65
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    A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed for the raspberry ketone in raspberry. Raspberry ketone was extracted by hot refluxing method with methanol as extraction solvent. The purities in crude extract was removed by chemical method, and the aldehyde, ketone and other carbonyl compounds were isolated from crude extract. Qualitative and quantitative analysis was carried out for the analyte under the MRM mode after the chromatographic separation on Symmetry C18 (2.0 mm×50 mm id, 2μ m) column. The quantitative limit of detection of RK in different matrix was 1.0 μ g kg-1. The average recovery was 75.4~82.3%, and the relative standard deviation was 5.97~8.40%. The method is accurate, rapid, sensitive, and adapt to the inspection of the raspberry ketone in raspberry. View full abstract»

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  • Survivin siRNA suppresses glioblastoma growth by inducing cell apoptosis

    Page(s): 66 - 71
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    To explore the effect of Survivin siRNA on U251 and C6 cells proliferation and apoptosis in vitro and rat glioma in vivo. Both cells lines were transfected with plasmids. Survivin, Bcl-2, c-Myc, cyclin D1, and caspase3 expression in U251 cells were analyzed by Western blot. The cell proliferation and apoptosis were determined by MTT, flow cytometry, and AO/EB assays. C6 glioma model was constructed in rat brain stereotactically. Tumor volumes were measured in time and growth curves were plotted. We found that Survivin expression was significantly reduced in Survivin siRNA transfected cells and apoptosis was induced greatly. Survivin siRNA decreased Bcl-2, cyclin D1 and c-Myc expression levels but promoted Caspase3 expression. Compared with the controlled groups, Survivin siRNA significantly suppressed the growths of C6 glioma intracranially and increased apoptosis rates in tissue but decrease Ki67 expression. In short, this study demonstrated that Survivin siRNA effectively suppressed growth and induced apoptosis, it also inhibited rat glioma growth intracranially. View full abstract»

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  • Systemic pelvic and paraaortic lymphadenectomy and prognostic significance in advanced epithelial ovarian malignancies: A meta-analysis and research

    Page(s): 72 - 75
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    We designed to evaluate the influence of pelvic and paraaortic lymphadenectomy in the prognosis of advanced epithelial ovarian cancers. The test group was treated with lymphadenectomy after cytoreductive surgery and the control group was treated only with cytoreductive surgery. The 3-year overall survival (OS), 5-year OS, and incidence of perioperative complications were considered as index. A total of 5 studies involving 899 patients met the inclusion criteria. Comparison revealed the following: there was no significant difference for the 3-year OS (RR = 1.06, 95% confidence interval (CI): 0.94~1.20, P = 0.35) and 5-year OS (RR = 1.17, 95%CI: 0.98~1.39, P = 0.08); incidence of perioperative complications of the test group was significantly higher (RR = 1.58, 95%CI: 1.15~2.18, P = 0.005) than that of control group. In conclusion, pelvic and paraaortic lymphadenectomy after cytoreductive surgery cannot significantly improve the patient survival, but can significantly increase the incidence of perioperative complications. View full abstract»

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  • Identification and characterization of DNA fingerprint among Fritillaria cirrhosa D. Don and other species of Fritillarias

    Page(s): 76 - 79
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    Objective: To explore the characterization of DNA fingerprints among Fritillaria cirrhosa D. Don and other species, and to establish effective method to identify Fritillari cirrhosa D. Don from other species. Methods: Genomic DNAs were extracted from Fritillaria cirrhosa D. Don and other species by the phenol method respectively. The specific primers for Fritillaria cirrhosa D. Don were designed by Primer Primer 5.0 according to the 5srDNA sequence in GenBank (Acession No. AF 2303201.1), and PCR technique (Polymerase Chain Reaction) was carried out to amplify the amplicon from the Fritillaria cirrhosa D. Don and others species. Results: The complete 19.3 kb DNA fragments were obtained from the Fritillaria cirrhosa D. Don, F. prezwalskii Maxim, F. unibracteata Hsiao et K.C. Hsia. Only fragment with 274 bp was amplified from the Fritillaria cirrhosa D. Don. Conclusion: The DNA fingerprint of Fritillaria cirrhosa D. Don showed the specific characteristics, which could be used to identify Fritillaria cirrhosa D. Don from others species. The method is an effective, simple, rapid and reliable in identifying the Fritillaria cirrhosa D. Don from others species. View full abstract»

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  • Research on the function of human embryo chorion promoting proliferation of cord blood CD34+ cells

    Page(s): 80 - 83
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    In many researches hematopoietic growth factors are used to stimulate the proliferation of cord blood CD34+ cells. But the proliferation quantity is not ideal enough, the cells are easily differentiated downwards. In this research human embryo chorion(HEC) is used as the culture medium for the observation of its functions in the proliferation of cord blood CD34+ cells. We isolated CD34+ cells from cord blood and cultured it using HEC as the culture medium to measure the quantity, purity and colony forming of CD34+ cells. It shows the 3 indexes of HEC group are all significantly higher than those of HGF group. These data implies HEC has the function of promoting the proliferation of cord blood CD34+ cells. Thus, this research effectively solves the problem of the shortage in cord blood CD34+ cells prior to the transplantation, which enables the wide application of cord blood CD34+ cells transplantation in adults. View full abstract»

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  • Application of the new evaluation methods in cancer diagnosis based on serum tumor markers data

    Page(s): 84 - 87
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    To set up a new evalution method in diagnosis of different carcinomas through positive index (PI), sum of direct positive index (SDPI) and sum of indirect positive index (SIPI). PI was defined as a ratio of resulting value (RV) to reference value (RF) in the article. Statistics was carried out to calculated test results of serum tumor markers. With its difference from sensitivity, specificity and accuracy, the three indexes represented different tumors and related diseases. For those diseases which could not be differentiated by positive rates, the SDPI and SIPI would be used to distinguish a varieties of diseases with similar symptoms and provide better references in diagnosis of different carcinomas clinically. View full abstract»

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  • Synthesis of vitcamphor derivatives of camphor and its preliminary anti-inflammatory activity

    Page(s): 88 - 91
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    The vitcamphor was synthesized from camphor as starting materials via the bromination, followed by esterification, reduction, hydrolisis and oxidation. The structure of the target compound was confirmed by 1H NMR and 13C NMR, and the overall yield was increased from 6% to 12%. The purity of the tide compound was over 96 %. The anti-inflammatory activity of vitcamphor was determined by vascular permeability of mice and rat carrageenin induced edema model. The vitcamphor in used doses exhibited remarkable inhibitory effect on histamine induced increase of vascular permeability and significantly reduced the paw swelling. View full abstract»

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