By Topic

Off-Axis Photoacoustic Microscopy

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Shelton, R.L. ; Dept. of Biomed. Eng., Texas A&M Univ., College Station, TX, USA ; Applegate, B.E.

Photoacoustic microscopy (PAM) is a high-contrast, high-resolution imaging modality, used primarily for imaging hemoglobin and melanin. Important applications include mapping of the microvasculature and melanoma tumor margins. We demonstrate a novel PAM design that markedly simplifies the implementation by separating the optical illumination from the acoustic detection path. This modification enables the use of high-quality commercial optics and transducers, and may be readily adapted to commercial light microscopes. The designed PAM system is only sensitive to signals generated in the overlap of the illumination and detection solid angles, providing the additional benefit of quasi-dark-field detection. An off-axis PAM system with a lateral resolution of 26 μm and a modest axial resolution of 410 μ m has been assembled and characterized using tissue samples. The axial resolution is readily scaled down to tens of micrometers within the same design, by utilizing commercially available high-frequency acoustic transducers.

Published in:

Biomedical Engineering, IEEE Transactions on  (Volume:57 ,  Issue: 8 )