By Topic

All Solid-State Potentiometric Biosensors for Creatinine Determination Based on pH and Ammonium Electrodes

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Nien-Hsuan Chou ; Inst. of Electron. Eng., Chung Yuan Christian Univ., Chungli ; Jung-Chuan Chou ; Tai-Ping Sun ; Shen-Kan Hsiung

This work investigates a disposable creatinine potentiometric biosensor based on a pH-sensitive electrode and an ammonium ion-selective electrode. An enzyme, creatinine iminohydrolase (CIH) was immobilized directly onto the surface of the electrodes by the entrapment method. According to the experimental results, the creatinine biosensors based on the pH-sensitive electrode and ammonium ion-selective electrode show a rapid response time (t95 < 30 s) and a linear dynamic response range for creatinine determination between 40 muM and 140 muM. Furthermore, the ammonium ion-selective electrode-based biosensors exhibited good operational stability (over 14 times) and storage stability (over 40 days). By discussing the reproducibility and pH effects of the creatinine biosensors, features of creatinine biosensors based on ammonium ion-selective electrodes are described. Moreover, development of a low-cost, solid-state, disposable potentiometric creatinine biosensor based on pH-sensitive electrodes and ammonium ion-selective electrodes is discussed.

Published in:

Sensors Journal, IEEE  (Volume:9 ,  Issue: 6 )