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We propose a segmentation algorithm for the purposes of large-scale flower species recognition. Our approach is based on identifying potential object regions at the time of detection. We then apply a Laplacian-based segmentation, which is guided by these initially detected regions. More specifically, we show that 1) recognizing parts of the potential object helps the segmentation and makes it more robust to variabilities in both the background and the object appearances, 2) segmenting the object of interest at test time is beneficial for the subsequent recognition. Here we consider a large-scale dataset containing 578 flower species and 250,000 images. This dataset is developed by our team for the purposes of providing a flower recognition application for general use and is the largest in its scale and scope. We tested the proposed segmentation algorithm on the well-known 102 Oxford flowers benchmark  and on the new challenging large-scale 578 flower dataset, that we have collected. We observed about 4% improvements in the recognition performance on both datasets compared to the baseline. The algorithm also improves all other known results on the Oxford 102 flower benchmark dataset. Furthermore, our method is both simpler and faster than other related approaches, e.g. [3, 14], and can be potentially applicable to other subcategory recognition datasets.