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Molecular-Level Dengue Fever Diagnostics: Developing a Combination of RT-LAMP and Paper-Based Devices

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5 Author(s)
Shih-Jie Lo ; Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, 300, Taiwan ; Shih-Chun Yang ; Da-Jeng Yao ; Jiann-Hwa Chen
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This article describes the development of an inexpensive but robust and easy-to-use point-of-care diagnostic device that can be used for the detection of dengue fever at the molecular level (i.e., the detection of dengue virus using a piece of paper). To date, the clinical diagnosis of dengue fever mainly relies on enzyme-linked immunosorbent assay-based examinations for a specific antigen. However, protein-based diagnostics are used at a relatively late stage of the infection, and there is a need to develop a simple and low-cost diagnostic device for the detection of dengue fever at the early stages of the infection.

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IEEE Nanotechnology Magazine  (Volume:6 ,  Issue: 4 )