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Currently the existing computer analysis system for drug discovery does not take it in to account that when cells are examined microscopically, excitation light or phosphor that is used on this occasion affects them adversely. We focused attention on phase contrast microscope which can observe cells non-invasively. Until now, phase contrast image has been difficult to process because recognition rate varies according to conditions, and for this, appropriate analyzing system has not been established. The study succeeded location of almost all cells.