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Microbial Fuel cells (MFCs) are batteries driven by bacteria. MFCs have the potential of powering small sensors in remote areas and disposing organic waste safely by harvesting the energy stored in the waste products. This paper describes the design of a novel single chamber MFC (SMFC) with carbon electrodes. Experiments were conducted to evaluate the performance of the MFC in power production. It is shown here that this SCMFC can generate electrical current without the use of PEM membranes or additives; the maximum voltage of around 411 mV can be achieved at the room temperature. These results also measured a various parameters such as pH, dissolved oxygen and solution conductivity during the operation of SMFC.